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Genome-wide chromatin accessibility profiling of Prdm12-edited CD8+ T cells by ATAC-seq

GSE301907 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/08/20 Platform GPL24247
Summary
This study investigates the impact of Prdm12 knockout on chromatin accessibility in CD8+ T cells. CD8+ T cells were isolated from spleens of OTI:Cas9 transgenic mice (C57BL/6 background) using magnetic bead-based negative selection. Isolated cells were subjected to CRISPR-Cas9 mediated gene editing targeting Prdm12 (Prdm12-KO group) or a non-targeting control guide RNA (Control group) via ribonucleoprotein (RNP) complex electroporation. ATAC-seq was performed to compare genome-wide chromatin accessibility between edited (treatment) and non-edited (control) cells, aiming to identify regulatory regions associated with Prdm12 function.
Published in
Prdm12 governs an epigenetic checkpoint linking neuroimmune cross-talk to CD8(+) T cell exhaustion-suppressed antitumor immunity
Liu G, Tian X, Wang Q et al. · Science advances 2025 · PMID 40815657 · doi:10.1126/sciadv.adx9221
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Also filed as BioProject PRJNA1287740 and SRA study SRP599230. Searching any of these in the dataset finder brings you back here.

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