Transcriptomic profiling of chlorogenic acid and taurine treatment in human skin cells provides insights into cellular senescence mechanisms

Chlorogenic acid (CGA) and taurine are well-known antioxidant compounds that have been demonstrated to reduce cellular senescence in the skin. Given their potential synergistic effects, co-treatment with CGA and taurine may enhance their individual efficacy. However, the biological mechanisms underlying their skin-protective effects remain underexplored. This study aimed to identify genes responsive to CGA and taurine and elucidate their anti-senescence-associated pathways. To this end, we conducted transcriptome-wide RNA sequencing to profile gene expression changes of human epidermal keratinocytes, melanocytes, and fibroblasts by treatment of CGA, taurine, and their combination. The differential expression analysis identified 197 differentially expressed genes (DEGs) in response to the compounds, of which 62 were prioritized as aging-related DEGs based on their involvement in skin aging pathways from functional enrichment analysis and supporting evidence from public databases and prior studies. By identifying key genes and pathways that might contribute to the cellular longevity in human skin, this study provides molecular insights for developing anti-aging strategies with potential applications in dermatology.