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Investigating Cycle-to-Cycle Variation and the Impact of Endometrial Injury through Differential Gene Expression Analysis

GSE303049 Homo sapiens (human) RNA-seq (gene expression) 40 samples Submitted 2026/05/15 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 40 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
40 samples: 19 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Investigating cycle-to-cycle variation and the impact of endometrial injury through differential gene expression analysisHuman reproduction (Oxford, England) · 2026
Summary (written by the submitters)
This prospective study investigated whether endometrial gene expression changes across two consecutive menstrual cycles and whether prior endometrial injury alters molecular profiles during the mid-luteal phase. Twenty women underwent paired endometrial biopsies, and differential gene expression was analysed using a paired design, adjusting for cycle timing.
Read the full summary ↓
While samples from the same individual showed slightly greater similarity than those from different individuals, no significant gene expression changes were observed after endometrial injury (p > 0.05), including in subgroup analyses of fertile and infertile participants. Principal component analysis showed no clear clustering by individual, further highlighting cycle-to-cycle variability. These findings suggest that intra-individual molecular variability is largely due to biological fluctuations rather than any lasting effect of endometrial injury. This may help explain inconsistent outcomes in implantation and limited utility of interventions like endometrial injury and molecular receptivity testing in IVF.
Published in
Investigating cycle-to-cycle variation and the impact of endometrial injury through differential gene expression analysis
Teh WT, Chung J, Donoghue JF et al. · Human reproduction (Oxford, England) 2026 · PMID 41770874 · doi:10.1093/humrep/deag025
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303049_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1293415 and SRA study SRP601842. Searching any of these in the dataset finder brings you back here.

Study design
19 conditions, mostly in duplicate
A ×2 B ×2 C ×2 D ×2 E ×2 F ×2 G ×2 H ×2 +13 more

Supports a between-group comparison across 38 samples.

How we read this: 19 replicated groups read from 40 sample titles; they account for 38 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.