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SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression program

GSE303179 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2025/08/01 Platform GPL24676Platform GPL34475Platform GPL34295
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples: 8 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression programMolecular biology of the cell · 2025
Summary (written by the submitters)
Alternative splicing expands proteomic diversity and is tightly regulated by splicing factors, including the serine/arginine-rich (SR) protein family. Here, we analyze the poorly characterized protein SRSF12. Although SRSF12 is conserved across vertebrates, it is poorly expressed in most mammals, and we find that SRSF12 knockouts in mice do not result in overt physiological or transcriptomic alterations.
Read the full summary ↓
In contrast, SRSF12 is more highly expressed in primates where it is predominantly expressed during meiosis and in the brain. SRSF12 localizes to splicing speckles in cultured human cells and interacts with the core splicing factors. Strikingly, ectopic expression of SRSF12 in human cells induces widespread transcriptional changes, activating testis- and brain-specific gene expression programs. SRSF12 overexpression also leads to mitotic arrest and cell death, phenotypes that require both its structured RNA recognition motif and intrinsically disordered arginine/serine-rich C-terminal domain. Together, our results suggest that SRSF12 has evolved primate-specific expression to regulate testis- and brain-specific genes.
Published in
SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression program
Ly J, Cady SL, Haug S et al. · Molecular biology of the cell 2025 · PMID 41060796 · doi:10.1091/mbc.E25-07-0352
This dataset
Open GSE303179 in finder → Find more RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303179_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1294295 and SRA study SRP602404. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41060796) ↗
Study design
8 conditions, mostly in duplicate
GFP_cJL220 ×3 SRSF12_GFP_cJL310 ×3 BL6_brain ×2 BL6_testis ×2 SRSF12KO_2nt_brain ×2 SRSF12KO_2nt_testis ×2 SRSF12KO_5nt_brain ×2 SRSF12KO_5nt_testis ×2

Supports a between-group comparison across 18 samples.

How we read this: 8 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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