Comparative Transcriptomic Analysis of Embryonic Hearts in Atf4 Exon 2 and Exon 2–3 Conditional Knockout Mice

This dataset comprises RNA sequencing (RNA-seq) profiles of E11.5 embryonic hearts from two Atf4 conditional knockout (cKO) mouse models, targeting different exons of the gene. In one model, exons 2 and 3 were floxed and excised in cardiomyocytes using the Xmlc2-Cre driver, while the second model involved deletion of exon 2 only. The aim of this study is to determine whether Atf4 exon-targeting strategy influences transcriptomic outcomes and to evaluate the impact of ATF4 deficiency on early cardiac development. RNA-seq was performed on ventricular tissues from both models and their respective littermate controls, each with four biological replicates. The dataset serves to clarify the necessity and function of ATF4 in the embryonic heart using refined conditional knockout strategies.