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GM-CSF-derived DCs from BM and BM progenitors and Spleen(SP) in vitro

GSE304117 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2026/05/27 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
CD32b defines distinct dendritic cell lineages generated from the culture of bone marrow with GM-CSFFrontiers in immunology · 2026
Summary (written by the submitters)
To investigate the heterogeneity of BM GM-DCs using progenitor-derived GM-DCs
Published in
CD32b defines distinct dendritic cell lineages generated from the culture of bone marrow with GM-CSF
Choi W, Ryu SH, Park JS et al. · Frontiers in immunology 2026 · PMID 42292399 · doi:10.3389/fimmu.2026.1703978
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE304117_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1298938 and SRA study SRP604688. Searching any of these in the dataset finder brings you back here.

Study design
6 conditions, mostly in triplicate
BM GM-DCs (day 7) ×3 BM GM-DCs (day 21) ×3 MDP GM-DCs (day 7) ×3 MDP GM-DCs (day 21) ×3 GMP GM-DCs (day 21) ×3 SP GM-DCs (day 21) ×3

Supports a between-group comparison across 18 samples.

How we read this: 6 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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