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Mouse placentae generated by in vitro fertilization exhibit altered gene expression, activated hypoxia responses and reduced fitness.

GSE303258 Mus musculus (mouse) Single-nucleus RNA-seq 96 samples Submitted 2025/08/08 Platform GPL24247
At a glance

Single-nucleus RNA-seq · mouse · 96 samples

What it is
Single-nucleus RNA-seqGene activity measured in individual nuclei (used when whole cells are hard to isolate, for example from frozen tissue). Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism
Mus musculus (mouse)
Samples
96 samples: 48 × snRNAseq-mouse placenta-FB vs 48 × snRNAseq-mouse placenta-IVFGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Mouse placentae generated by in vitro fertilization exhibit altered gene expression, activated hypoxia responses, and reduced fitness†Biology of reproduction · 2026
Summary (written by the submitters)
In this study, we utilized single-nucleus RNA sequencing to quantify alterations in the gene expression programs of mouse placentas conceived through in vitro fertilization (IVF). We identified genetic programs exhibiting both global and cell type specific differences between IVF and natural in vivo fertilization groups. Gene set enrichment analysis revealed pathways associated with parietal trophoblast giant cell differentiation and implicated in the regulation of lactation (placental lactogens), along with Hypoxia-inducible factor dependent gene expression.
Read the full summary ↓
Importantly, IVF-derived conceptuses showed increased abortion rates when their surrogate mothers were exposed to hypoxia (10.5% O2) during pregnancy (from E7.5 to 12.5). Collectively, our findings shed light on the cellular and molecular underpinnings driving differences in pregnancy outcomes associated with these conception methods and indicate that IVF can sensitize embryos to additional stressful events, as proposed by the developmental origin of health and disease hypothesis.
Published in
Mouse placentae generated by in vitro fertilization exhibit altered gene expression, activated hypoxia responses, and reduced fitness†
Oqani RK, Maltepe E, Rinaudo P et al. · Biology of reproduction 2026 · PMID 40874534 · doi:10.1093/biolre/ioaf193
This dataset
Open GSE303258 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303258_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1292283 and SRA study SRP601803. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40874534) ↗
Study design
48 × snRNAseq-mouse placenta-FB vs 48 × snRNAseq-mouse placenta-IVF

Supports a between-group comparison across 96 samples.

How we read this: 2 replicated groups read from 96 sample titles; they account for 96 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 56 more — browse all 96 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.