GEO series

Tmem45b Modulates itch via Endoplasmic Reticulum Calcium Regulation

GSE304359 Mus musculus (mouse) RNA-seq (gene expression) 21 samples Submitted 2026/01/01 Platform GPL28330

At a glance

RNA-seq (gene expression) · mouse · 21 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 21 samples: 11 × Mrgprd-cre mice, RNA-seq, vs 6 × DRG, Tmem45b-flox mice, RNA-seq, vs 4 × DRG, CFA model，Tmem45b-flox mice, RNA-seq,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Chronic itch is detected by dorsal root ganglia (DRG) that severely impairs quality of life. However, its underlying mechanisms remain poorly understood. We identified that Tmem45b was contained in natriuretic peptide type B (Nppb)-, mas-related G-protein coupled receptor member A3 (Mrgpra3)-, and Mrgprd-positive DRG neurons, which are associated with itch sensation.

Read the full summary ↓

The role of Tmem45b in itch sensation has not been explored. Tmem45b conditional knockout (cKO) mice exhibited decreased scratching to β-alanine, and increased scratching to chloroquine. Notably, Tmem45b cKO alleviated chronic itch. Furthermore, Tmem45b cKO impaired the calcium response to β-alanine and allyl isothiocyanate but not to chloroquine in dissociated DRG neurons. Tmem45b deficiency led to significant downregulation of sarco/endoplasmic reticulum calcium transport ATPase 1 (Serca1), impairing calcium storage capacity of endoplasmic reticulum (ER). Inhibition of Serca1 in DRG neurons reduced intracellular calcium release triggered by β-alanine and chloroquine. Together,Tmem45b deficiency may reduce nonhistaminergic itch and disrupt ER calcium regulation, highlighting a potential target for chronic itch therapy.

This dataset

Open GSE304359 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE304359_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1300793 and SRA study SRP606009. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

11 × Mrgprd-cre mice, RNA-seq, vs 6 × DRG, Tmem45b-flox mice, RNA-seq, vs 4 × DRG, CFA model，Tmem45b-flox mice, RNA-seq,

Supports a between-group comparison across 21 samples.

How we read this: 3 replicated groups read from 21 sample titles; they account for 21 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9149270 DRG, CFA model，Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep1 Mrgprd-cre mice, RNA-se…
GSM9149271 DRG, CFA model，Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep2 Mrgprd-cre mice, RNA-se…
GSM9149272 DRG, CFA model，Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep3 Mrgprd-cre mice, RNA-se…
GSM9149273 DRG, CFA model，Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep4 Mrgprd-cre mice, RNA-se…
GSM9149274 DRG, CFA model，Tmem45b-flox mice, RNA-seq, rep1 DRG, CFA model，Tmem45b-…
GSM9149275 DRG, CFA model，Tmem45b-flox mice, RNA-seq, rep2 DRG, CFA model，Tmem45b-…
GSM9149276 DRG, CFA model，Tmem45b-flox mice, RNA-seq, rep3 DRG, CFA model，Tmem45b-…
GSM9149277 DRG, CFA model，Tmem45b-flox mice, RNA-seq, rep4 DRG, CFA model，Tmem45b-…
GSM9149278 DRG, Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep1 Mrgprd-cre mice, RNA-se…
GSM9149279 DRG, Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep2 Mrgprd-cre mice, RNA-se…
GSM9149280 DRG, Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep3 Mrgprd-cre mice, RNA-se…
GSM9149281 DRG, Tmem45b-flox mice, RNA-seq, rep1 DRG, Tmem45b-flox mice,…
GSM9149282 DRG, Tmem45b-flox mice, RNA-seq, rep2 DRG, Tmem45b-flox mice,…
GSM9149283 DRG, Tmem45b-flox mice, RNA-seq, rep3 DRG, Tmem45b-flox mice,…
GSM9149284 DRG, Tmem45b-flox mice, RNA-seq, rep4 DRG, Tmem45b-flox mice,…
GSM9149285 DRG, Tmem45b-flox mice, RNA-seq, rep5 DRG, Tmem45b-flox mice,…
GSM9149286 DRG, Tmem45b-flox mice, RNA-seq, rep6 DRG, Tmem45b-flox mice,…
GSM9149287 DRG, Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep4 Mrgprd-cre mice, RNA-se…
GSM9149288 DRG, Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep5 Mrgprd-cre mice, RNA-se…
GSM9149289 DRG, Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep6 Mrgprd-cre mice, RNA-se…
GSM9149290 DRG, Tmem45b-flox::Mrgprd-cre mice, RNA-seq, rep7 Mrgprd-cre mice, RNA-se…

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