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N,N Dimethylacetamide attenuates LPS induced neuroinflammation in a murine model

GSE304500 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/02/17 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Neuroinflammation is a central driver of cognitive decline in many neurodegenerative diseases and is marked by persistent activation of pro-inflammatory pathways. N,N-Dimethylacetamide (DMA), an FDA-approved excipient with emerging anti-inflammatory properties, was evaluated for its therapeutic potential in a murine model of chronic neuroinflammation.
Read the full summary ↓
Both male and female C57BL/6 mice were administered lipopolysaccharide (LPS; 0.5 mg/kg) to induce neuroinflammation over seven days, followed by treatment with DMA. Behavioral assays—including the Radial Arm Maze and Novel Object Recognition tests—revealed significant cognitive deficits in LPS-treated animals, which were attenuated in DMA-treated mice, indicating preserved spatial memory and object recognition. To elucidate underlying molecular changes, RNA sequencing was performed on brain tissue, and differential gene expression analysis was followed by KEGG pathway enrichment. LPS exposure upregulated pathways related to immune activation, including TNF signaling, NF-κB signaling, MAPK signaling, and cytokine–cytokine receptor interactions. DMA treatment reversed many of these transcriptional changes, downregulating pro-inflammatory signaling and upregulating genes involved in neuroprotection and synaptic signaling. These findings suggest that DMA not only modulates key inflammatory pathways at the transcriptomic level but also improves cognitive function in a neuroinflammatory context. Collectively, this study highlights DMA’s potential as a therapeutic agent for preventing inflammation-associated cognitive decline.
This dataset
Open GSE304500 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE304500_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1301391 and SRA study SRP606426. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
6 conditions, mostly in duplicate
Brain Tissue_Control group_Female ×2 Brain Tissue_Disease Group_Female ×2 Brain Tissue_ Treatment Group_Female ×2 Brain Tissue_Control group_Male ×2 Brain Tissue_Disease Group_Male ×2 Brain Tissue_ Treatment Group_Male ×2

Supports a case/control comparison: 4 samples read as cases, 4 as controls.

How we read this: 6 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.