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Hybrid Endometrial-Derived Hydrogel and Human Endometrial Organoids Synergize for Uterine Regeneration in an Immunocompetent Murine Model

GSE304635 Mus musculus (mouse) RNA-seq (gene expression) 23 samples Submitted 2025/12/11 Platform GPL21626
At a glance

RNA-seq (gene expression) · mouse · 23 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
23 samples: 6 × SHAM_S vs 6 × HG_S vs 6 × HG+ORG_S vs 5 × PBS_SGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Hybrid endometrial-derived hydrogel and human endometrial organoids synergize for uterine regeneration in an immunocompetent murine modelStem cell research & therapy · 2026
Summary (written by the submitters)
Study question: Can human endometrial organoids (hEOs) synergize with a hybrid endometrial-derived hydrogel (HG) to regenerate the endometrium in an immunocompetent murine model of Asherman’s Syndrome/Endometrial Atrophy (AS/EA)? Summary answer: The combination of HG+hEOs was biocompatible in an immunocompetent murine model, restored endometrial thickness and glandular structure, reduced fibrosis and ferroptosis, promoted angiogenesis and regenerative immune pathways, but failed to fully restore fertility, likely due to the prolonged retention of the synthetic hydrogel component.
Read the full summary ↓
Main results and the role of chance: No systemic immune alterations or hematological abnormalities were detected post-treatment, indicating that the combined treatment was compatible and safe in mice. Fourteen days after treatment, HG+hEOs treatment significantly restored endometrial thickness and gland density, reduced collagen deposition and iron accumulation (ferroptosis), and enhanced vascularization compared to the PBS condition (p < 0.05), reaching levels comparable to SHAM controls. RNA sequencing of the murine endometria confirmed restoration of transcriptomic profiles and upregulation of angiogenesis, cell proliferation, and regenerative immune pathways in the HG+hEOs group. However, fertility outcomes (pregnancy rate and embryo number) remained significantly impaired in all conditions, with no statistical improvement over the PBS group. Mice injected with PM confirmed that fertility impairment was linked to the presence of synthetic hydrogel in previously damaged uteri.
Published in
Hybrid endometrial-derived hydrogel and human endometrial organoids synergize for uterine regeneration in an immunocompetent murine model
Gómez-Álvarez M, Bueno-Fernandez C, Francés-Herrero E et al. · Stem cell research & therapy 2026 · PMID 41526996 · doi:10.1186/s13287-026-04900-1
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE304635_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1301962 and SRA study SRP606705. Searching any of these in the dataset finder brings you back here.

Study design
6 × SHAM_S vs 6 × HG_S vs 6 × HG+ORG_S vs 5 × PBS_S

Supports a between-group comparison across 23 samples.

How we read this: 4 replicated groups read from 23 sample titles; they account for 23 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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