GEO series

Autophagy acts as a brake on obesity-related fibrosis by controlling purine nucleoside signalling

GSE306424 Mus musculus (mouse) RNA-seq (gene expression) 10 samples Submitted 2025/09/20 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 10 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 10 samples: 4 × XANTH vs 3 × CTRL vs 3 × HYPOXGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Autophagy acts as a brake on obesity-related fibrosis by controlling purine nucleoside signallingNature communications · 2025

Summary (written by the submitters)

A hallmark of obesity is a pathological expansion of white adipose tissue (WAT), accompanied by marked tissue dysfunction and fibrosis. Autophagy promotes adipocyte differentiation and lipid homeostasis, but its role in obese adipocytes and adipose tissue dysfunction remains incompletely understood. Using a mouse model, we demonstrate that autophagy is a key tissue-specific regulator of WAT remodelling in diet-induced obesity.

Read the full summary ↓

Importantly, loss of adipocyte autophagy substantially exacerbates pericellular fibrosis in visceral WAT. Change in WAT architecture correlates with increased infiltration of macrophages with tissue-reparative, fibrotic features. We uncover that autophagy restrains purine nucleoside metabolism in obese adipocytes. This ultimately leads to a reduced release of the purine catabolites xanthine and hypoxanthine. Purines signal cell-extrinsically for fibrosis by driving macrophage polarisation towards a tissue reparative phenotype. Our findings in mice reveal a novel role for adipocyte autophagy in regulating tissue purine nucleoside metabolism, thereby limiting obesity-associated fibrosis and maintaining the functional integrity of visceral WAT. Purine signals may serve as a critical balance checkpoint and therapeutic target in fibrotic diseases

Published in

Autophagy acts as a brake on obesity-related fibrosis by controlling purine nucleoside signalling

Piletic K, Kayvanjoo AH, Richter FC et al. · Nature communications 2025 · PMID 41107256 · doi:10.1038/s41467-025-64266-5

This dataset

Open GSE306424 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE306424_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1310606 and SRA study SRP612451. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41107256) ↗

Study design

4 × XANTH vs 3 × CTRL vs 3 × HYPOX

Supports a between-group comparison across 10 samples.

How we read this: 3 replicated groups read from 10 sample titles; they account for 10 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9199144 CTRL1 CTRL
GSM9199145 CTRL2 CTRL
GSM9199146 CTRL3 CTRL
GSM9199147 HYPOX1 HYPOX
GSM9199148 HYPOX2 HYPOX
GSM9199149 HYPOX4 HYPOX
GSM9199150 XANTH1 XANTH
GSM9199151 XANTH2 XANTH
GSM9199152 XANTH3 XANTH
GSM9199153 XANTH4 XANTH

Datasets on a similar topic

GSE282477 PLAC8 attenuates pulmonary fibrosis and inhibits apoptosis of alveolar epithelial cells via facilitating autophagy 11 samples shares autophagy, fibrosis
GSE292735 Defective autophagy in CD4 T cells drives liver fibrosis via type 3 inflammation 11 samples shares autophagy, fibrosis
GSE265909 yHSC and oHSC scRNAseq with oHSC AThi ATlo Index Sorting. Assessing the transcriptional changes associated with autophagy engagement in old hematopoietic stem cells at single cell resolution. [scRNA-Seq] 563 samples shares autophagy
GSE269192 ZEB2 is a Master Switch Controlling the Tumor-Associated Macrophage Program 493 samples shares controlling
GSE269188 ZEB2 is a Master Switch Controlling the Tumor-Associated Macrophage Program [KO_screen] 331 samples shares controlling
GSE269191 ZEB2 is a Master Switch Controlling the Tumor-Associated Macrophage Program [in vivo] 145 samples shares controlling
GSE278523 Systems biology mapping of rBCG-LTAK63-induced protection against tuberculosis reveals the significance of autophagy and circadian rhythm regulation by cAMP 126 samples shares autophagy
GSE275147 A catecholamine-independent pathway controlling adaptive adipocyte lipolysis 79 samples shares controlling

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.