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DAXX Directs H3.4-to-H3.3 Histone Replacement During Spermatogenesis [RNA-Seq]

GSE308003 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/01/13 Platform GPL34290
Summary
During spermatogenesis, extensive chromatin remodeling and histone replacement reshape the male germline epigenome. While HIRA is known to mediate transcription-coupled incorporation of histone variant H3.3, we identify DAXX as a key histone chaperone directing genome-wide, transcription-dependent replacement of H3.4 (H3T) with H3.3 on autosomes during male meiosis. Simultaneously, DAXX also directs transcription-independent H3.4-to-H3.3 replacement on the sex chromosomes during meiotic sex chromosome inactivation (MSCI). These distinct, chromosome-specific modes of DAXX-mediated H3.3 deposition are essential for epigenomic integrity in the male germline. Loss of DAXX disrupts this process, leading to widespread transcriptional dysregulation in haploid round spermatids and male infertility.
Published in
DAXX directs dual modes of H3.4-to-H3.3 histone replacement in the male germline
Yeh YH, Hu M, Otsuka K et al. · Genes & development 2026 · PMID 41980770 · doi:10.1101/gad.353435.125
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Also filed as BioProject PRJNA1328501 and SRA study SRP620314. Searching any of these in the dataset finder brings you back here.

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