LRRK2-mediated neuroinflammation induced neuronal dysfunctions in a Parkinson’s and Alzheimer’s disease cellular model

Dissect the transcriptional consequences of neuroinflammation and the role of the kinase LRRK2 in regulating neuronal responses in human induced pluripotent stem cell (hiPSC)-derived neurons exposed to pro-inflammatory conditions relevant to Parkinson’s disease (PD) and Alzheimer’s disease (AD).Dopaminergic neurons (PD context) and cholinergic neurons (AD context) were differentiated from hiPSCs and subsequently cultured in glial-conditioned medium containing either α-synuclein pre-formed fibrils (α-syn pffs) or amyloid-β (Aβ) fibrils, for PD and aAD respectively. Neuronal cultures were exposed to inflammed media to model chronic inflammatory stress.