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Exercise intensity differentially modulates systemic molecular responses and predicted interorgan communication in humans

GSE308252 Homo sapiens (human) RNA-seq (gene expression) 62 samples Submitted 2026/06/23 Platform GPL30173
At a glance

RNA-seq (gene expression) · human · 62 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
62 samples: 62 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Exercise is recognized as a first-line lifestyle therapy for many cardiometabolic diseases, including obesity, type 2 diabetes, and hypertension. Despite the abundant health-promoting effects of exercise, in-depth characterization of circulatory factors mediating these benefits remain understudied in humans. Moreover, how different modes of exercise uniquely regulate these processes is unclear.
Read the full summary ↓
Here, we address these questions by conducting a multi-cohort human exercise intervention, incorporating sprint-interval exercise (SIE), moderate-intensity exercise (MIE), and treadmill exercise to exhaustion to analyze intensity-dependent regulation of adipocytes and bulk adipose tissue. We find that exercise intensity uniquely influences the plasma proteome and plasma metabolome, and culturing primary human adipocytes with these plasma samples results in robust intensity-dependent transcriptome changes. We confirm many of these genes are similarly regulated by intense exercise in vivo, via RNA-sequencing of bulk adipose tissue following treadmill exercise to exhaustion. These findings underscore intensity-dependent regulation of adipose tissue following exercise.
This dataset
Open GSE308252 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE308252_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1329302 and SRA study SRP620862. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
62 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 62 sample titles: 62 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 22 more — browse all 62 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.