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Aberrant fibro-adipogenic progenitor subpopulations drive volumetric muscle loss-induced fibrosis

GSE308267 Mus musculus Expression profiling by high throughput sequencing; Other 6 samples Submitted 2025/09/18 Platform GPL21626
Summary
Volumetric muscle loss (VML) injuries result in chronic fibrosis, inflammation, and persistent functional deficits. Fibro-adipogenic progenitor (FAP) cells are a heterogeneous, muscle-resident stromal cell population that play a crucial role in muscle regeneration but also contribute to fibrosis in muscle disease. The role of FAPs in VML is not well established and may be a critical target to ensure functional muscle regeneration after VML. We utilized a critical VML model in the mouse quadriceps, compared with subcritical injuries and uninjured controls, to study the single-cell transcriptional profile of FAPs after VML. Our findings establish an aberrant FAP subpopulation that is elevated in VML injury and provide novel targets for future scarless muscle regeneration in VML.
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Direct links to NCBI, no account and no request form: the whole study as GSE308267_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1329328 and SRA study SRP620879. Searching any of these in the dataset finder brings you back here.

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