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RNA-seq profiling of mouse Sertoli cell line after siDhx37 knockdown

GSE309765 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/11/14 Platform GPL24247
Summary
Sertoli cells play a crucial role in supporting spermatogenesis in the testes. In this study, we aimed to investigate the molecular changes in Sertoli cells after the knockdown of Dhx37, a gene involved in RNA splicing and gene regulation. siDhx37 was transfected into the mouse Sertoli cell line, followed by RNA-seq analysis at Day 0 and Day 3 post-transfection. The results reveal that Dhx37 depletion affects the expression of key genes involved in Sertoli cell function, spermatogenesis, and cell cycle regulation. Differentially expressed genes (DEGs) were identified, highlighting pathways related to cellular processes, including cell proliferation, apoptosis. Our findings suggest that Dhx37 may play an important regulatory role in Sertoli cells by modulating the expression of genes necessary for their function in male reproduction.
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Direct links to NCBI, no account and no request form: the whole study as GSE309765_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1363150 and SRA study SRP644289. Searching any of these in the dataset finder brings you back here.

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