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NUDT21 knockdown RNA-seq in neuroblastoma II

GSE311302 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/07/01 Platform GPL34284
Summary
Alternative polyadenylation (APA) generates mRNAs with distinct 3′-untranslated regions (3′ UTRs) to regulate post-transcriptional gene expression and is critical in many tumors. However, the role of alternative 3′ UTRs in neurolastoma (NB) remains elusive. Here, we show that NUDT21 knockdown inhibits NB cell proliferation, induces cycle arrest/apoptosis in vitro, and suppresses xenograft growth in nude mice. Mechanistically, NUDT21 silencing triggers global APA dynamics, with ∼85% of APA genes switching to proximal poly(A) sites, shortening 3′ UTRs. Integrative PAS-seq/RNA-seq analyses reveal that NUDT21 regulates E2F targets (e.g., MKI67) via APA. Additionally, NUDT21 occupies gene regulatory regions to directly activate E2F target transcription. Our findings demonstrate NUDT21 sustains NB proliferation through dual APA-dependent and APA-independent mechanisms, highlighting targeting NUDT21 or its downstream pathways as a promising therapeutic strategy.
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Direct links to NCBI, no account and no request form: the whole study as GSE311302_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1368904 and SRA study SRP648694. Searching any of these in the dataset finder brings you back here.

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