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Next Generation Sequencing Facilitates Quantitative Analysis of the Role of Ddx21 in hematopoietic stem cells [RNA-seq]

GSE312007 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/03/20 Platform GPL24247
Summary
Hematopoietic stem cell (HSC) homeostasis is critically dependent on precisely regulated protein synthesis, with the nucleolus serving as the principal orchestrator of ribosome biogenesis and cellular stress sensing. DDX21, a nucleolus-localized DEAD-box RNA helicase, serves as a master regulator in ribosomal DNA (rDNA) transcription and ribosome assembly. Nevertheless, its precise function in adult hematopoiesis remains incompletely understood. Here, we reveal that conditional knockout of Ddx21 in the murine hematopoietic system triggers collapse of HSC homeostasis, characterized by loss of quiescence, impaired self-renewal capacity, and raised apoptosis. Mechanistically, we demonstrate that DDX21 physically associates with both rDNA loci and promoters of ribosomal protein genes. Its loss disrupts ribosome production, causes nucleolar stress, culminates in p53-mediated cell cycle arrest and apoptosis in HSPCs. Taken together, our study establishes DDX21 as a master regulator in HSCs, integrating ribosome biogenesis with nucleolar stress induction and hematopoietic homeostasis.
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Also filed as BioProject PRJNA1372051 and SRA study SRP650415. Searching any of these in the dataset finder brings you back here.

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