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Stag2-mediated chromatin dynamics regulates antibody class switch recombination [RNA-Seq]

GSE312230 Mus musculus (mouse) RNA-seq (gene expression) 9 samples Submitted 2026/05/20 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 9 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
9 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Stag2-mediated chromatin dynamics regulates antibody class switch recombinationProceedings of the National Academy of Sciences of the United States of America · 2026
Summary (written by the submitters)
Class switch recombination (CSR) in B lymphocytes generates different functional antibody isotypes and cohesin-mediated chromatin loop extrusion is proposed to promote CSR. However, the detailed regulatory mechanism of cohesin in CSR is largely lacking. Here, we revealed that Stag2 is a specific cohesin regulator playing critical roles in promoting CSR.
Read the full summary ↓
Stag2-deficiency, not Stag1-deficiency, significantly decreases the interaction of acceptor CH with CSR center, leading to decreased transcription of acceptor CH and impaired CSR. Notably, Stag2 could compensate for Stag1 binding within Igh upon Stag1-deficiency, while Stag2-deficiency significantly decreases Stag1 binding within Igh. Interestingly, Stag2 expression is higher than Stag1 during germinal center (GC) B cell development and Stag2 expression has a high correlation with CSR level in GC B cells upon vaccination and SARS-Cov-2 infection. Furthermore, Stag2 is also highly expressed in GC B cells within different tumors, corresponding to the high level of CSR in tumors. Altogether, our findings uncover the unrecognized specific roles of Stag2 in regulating CSR during physiological and pathological immune responses.
Published in
Stag2-mediated chromatin dynamics regulates antibody class switch recombination
Wan Z, Yu L, Yang Z et al. · Proceedings of the National Academy of Sciences of the United States of America 2026 · PMID 42085150 · doi:10.1073/pnas.2536391123
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE312230_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1372706 and SRA study SRP650771. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 9 more — browse all 9 samples with per-sample file links →

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