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Gene expression profiles at the single-nucleus level in heart tissue after myocardial infarction and saNppa-LNP treatment

GSE313956 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/03/20 Platform GPL24247
Summary
This project aims to investigate the cellular and molecular mechanisms underlying the therapeutic effects of a self-amplifying RNA encoding atrial natriuretic peptide (saNppa) in myocardial infarction (MI). In this study, a myocardial infarction model was established in C57BL/6 mice by coronary artery ligation. On the day of surgery, mice received intramuscular injection of saNppa formulated in lipid nanoparticles (saNppa-LNP) or phosphate-buffered saline (PBS) as control. Single-nucleus RNA sequencing (snRNA-seq) was performed on left ventricular tissue collected at Day 7 post-MI and treatment. Three experimental groups were included: sham-operated mice, MI mice treated with PBS (MI+PBS), and MI mice treated with saNppa-LNP (MI+saNppa-LNP). This dataset provides a comprehensive single-nucleus transcriptional landscape of the post-infarct heart and enables cell type–specific analysis of transcriptional changes associated with myocardial infarction and saNppa self-amplifying RNA therapy.
Published in
Single intramuscular injection of self-amplifying RNA of Nppa to treat myocardial infarction
Zhang K, Tao H, Zhu D et al. · Science (New York, N.Y.) 2026 · PMID 41785353 · doi:10.1126/science.adu9394
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Direct links to NCBI, no account and no request form: the whole study as GSE313956_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 3 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1387437 and SRA study SRP655655. Searching any of these in the dataset finder brings you back here.

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