Repertoire FLEX (REFLEX) Enables High-Throughput Multiomic scRNAseq with AIR Detection

Profiling the vast diversity of the T-cell repertoire at single-cell resolution is challenging due to the scale and cost requirements involved. Current technologies capture TCR or perform single-cell sequencing at scale but struggle to combine both. Here, we utilize both neat and virally stimulated PBMCs to showcase “REFLEX”, a technological approach enabling highly scalable, low cost-per-cell profiling of TCR repertoire in parallel with epitope and gene expression. This combination of capabilities allows for high throughput, multi-omic profiling of rare T cell subsets and longitudinal study formats at scales that were previously unattainable.