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RNA-seq of WT and Rbm25-deficient peritoneal macrophages infected with vesicular stomatitis virus (VSV)

GSE314891 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/07/15 Platform GPL24247
Summary
The purpose of this study is to detect differentially expressed genes in wild type (WT) and Rbm25-deficient peritoneal macrophages (PMs) infected with vesicular stomatitis virus for 4 hours. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Mouse PMs from WT and Rbm25-deficient mice were cultured in DMEM medium. PMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. WT and Rbm25-deficient PMs were infected with vesicular stomatitis virus for 4 hours, of which RNA profiles were generated by deep sequencing, using Illumina. We identified several differently expressed genes in the two groups of cells.
Published in
RNA-Binding Protein RBM25 Targets the mRNA Stability of GTPase Rab22a to Restrict Viral Entry and Infection
Ding Y, Chen H, Jiang Y et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 · PMID 42299815 · doi:10.1002/advs.76160
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Also filed as BioProject PRJNA1393090 and SRA study SRP657708. Searching any of these in the dataset finder brings you back here.

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