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RNA-seq analysis of bEnd.3 cells with shRNA-mediated knockdown of Npc1 versus control

GSE316088 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/03 Platform GPL28457
Summary
This study aims to elucidate the transcriptional regulatory role of the Npc1 gene in bEnd.3 cells through transcriptome sequencing. We achieved stable knockdown of Npc1 in bEnd.3 cells using transposon-delivered shRNA, while the control group received non-targeting shRNA (n=3 biological replicates per group). Knockdown efficiency was validated by qRT-PCR (reduction >90%). Total RNA was extracted and sequenced on the DNBSEQ platform. Differential expression analysis revealed that differentially expressed genes were significantly enriched in pathways related to lipid metabolism and tight junction function, indicating that Npc1 plays a critical role in regulating these cellular processes in bEnd.3 cells. This dataset provides a resource for studying Npc1-dependent gene networks.
Published in
Npc1 deficiency impairs brain vascular integrity in mouse model of Niemann-Pick disease type C1
Kang J, Gao G, Qiao L et al. · Neurobiology of disease 2026 · PMID 42208871 · doi:10.1016/j.nbd.2026.107462
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Also filed as BioProject PRJNA1399329 and SRA study SRP660411. Searching any of these in the dataset finder brings you back here.

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