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RXR agonist increases newly characterized GM-CSF-producing B220+CD8αα interepithelial T cells

GSE318304 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/19 Platform GPL24247
Summary
Context-dependent cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) plays a crucial role in maintaining intestinal immune homeostasis and exacerbating inflammation. Although multiple cell types produce GM-CSF in the gut, whether intraepithelial lymphocytes (IELs) contribute to GM-CSF production during the steady state remains unclear. In the small intestinal epithelial layer, we identified B220-expressing CD8αα T cells that selectively produced GM-CSF but not interleukin-17A or interferon-gamma. Transcriptional analysis combined with weighted parametric gene set analysis revealed the upregulation of retinoid X receptor (RXR)-related genes in this population. Consistently, the RXR agonist bexarotene increased GM-CSF-producing B220+CD8αα T cells without affecting other cytokines or CD8αβ T cells. These findings identify B220+CD8αα IELs as a spatially distinct source of GM-CSF in the epithelial layer and indicate that the RXR pathway specifically facilitates GM-CSF-producing program, potentially contributing to maintaining immune homeostasis in the small intestine.
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Also filed as BioProject PRJNA1418564 and SRA study SRP674415. Searching any of these in the dataset finder brings you back here.

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