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Single-cell RNA sequencing of alveolar macrophages in GRP75 knockdown mice with SARS-CoV-2 N protein-induced lung injury

GSE318978 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/06/01 Platform GPL24247
Summary
This study investigates the role of GRP75 (Hspa9)-mediated mitochondria-associated membranes (MAMs) in SARS-CoV-2 nucleocapsid (N) protein-induced acute lung injury. Using single-cell RNA sequencing of lung macrophages from control and GRP75 knockdown mice, we demonstrate that N protein promotes GRP75-dependent ER-mitochondria tethering, leading to mitochondrial calcium overload and proinflammatory macrophage activation. GRP75 knockdown attenuates N protein-induced lung injury by reprogramming alveolar macrophage identity from a pro-inflammatory Car4+ state to a metabolically quiescent Hspa9-low state.
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Direct links to NCBI, no account and no request form: the whole study as GSE318978_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 3 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1420915 and SRA study SRP675924. Searching any of these in the dataset finder brings you back here.

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