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Adipocytes signal to recruit specific mRNAs from surrounding cells to restore expression deficits [RNA-Seq]

GSE319160 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/03/03 Platform GPL34290
Summary
Extracellular vesicles (EVs) are nano-sized, membrane-delimited, particles released by cells that carry signaling macromolecules. A major pathway of EV production is potentiated by neutral sphingomyelinase 2 (SMPD3/nSMAse2), an enzyme that generates ceramide from sphingomyelin. In our attempt to study this pathway in adipocytes of male mice, we discovered that the elimination of SMPD3 from adipocytes in vivo triggers a signal to surrounding immune cell-like preadipocytes to release EVs that carry SMPD3 mRNA. This results in a widespread increase in SMPD3 mRNA in purified null adipocytes without a change in the transcripts of other enzymes involved in ceramide metabolism. These results point to a fundamental, novel, and selective mechanism by which specific mRNA molecules are acquired from the microenvironment to a level that can restore expression of mRNA and protein in a cell that is depleted of the corresponding genetic information.
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Direct links to NCBI, no account and no request form: the whole study as GSE319160_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1422231 and SRA study SRP676316. Searching any of these in the dataset finder brings you back here.

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