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MELK inhibition promotes megakaryopoiesis and platelet recovery in immune thrombocytopenia through ERK–cofilin signaling

GSE322507 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/04/02 Platform GPL21290
Summary
Maternal embryonic leucine zipper kinase (MELK) has been implicated in cell-cycle regulation, yet its transcriptional programs in megakaryocytic cells remain incompletely defined. To profile MELK-dependent gene expression changes, we performed bulk RNA-seq on the human megakaryoblastic cell line MEG-01 transduced with a control pLKO.1 vector or a MELK-targeting shRNA (sh2-MELK), with three independent biological replicates per condition. Paired-end sequencing reads (raw FASTQ files) and processed gene-level expression quantifications are provided to support differential expression and pathway analyses of MELK knockdown in megakaryocytic cells.
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Direct links to NCBI, no account and no request form: the whole study as GSE322507_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1430239 and SRA study SRP680165. Searching any of these in the dataset finder brings you back here.

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