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RNA-seq analysis of carbon framework-mediated metabolic reprogramming in senescent cells: effects of C3N nanosheets and photoactivation

GSE322654 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2026/03/04 Platform GPL34284
Summary
This RNA-seq dataset investigates the senolytic effects of carbon nitride quantum dots (C3N QDs) combined with irradiation on senescent human umbilical vein endothelial cells (HUVECs). The study compares three experimental conditions: senescence alone (S), senescence treated with C3N QDs (S+C), and senescence treated with C3N QDs followed by irradiation (S+C+I). Transcriptomic profiling revealed that the combined treatment (S+C+I) significantly reprogrammed gene expression patterns associated with aging and inflammation. Specifically, the S+C+I group showed a notable upregulation of genes such as MAPT, FOXO4, and PTEN, while markedly downregulating key senescence-associated secretory phenotype (SASP) factors including IL6, CXCL8, SERPINE1, and TERT compared to the S group. These data provide comprehensive insights into the molecular mechanisms by which C3N QDs-mediated photoactivation alleviates cellular senescence and suppresses pro-inflammatory pathways.
Published in
Structural evolution of carbon frameworks realizes in vitro interfacial transport in metabolically reprogrammed senescent cells for senolysis
Wang X, Ma H, Li Y et al. · Nature communications 2026 · PMID 41844622 · doi:10.1038/s41467-026-70810-8
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Direct links to NCBI, no account and no request form: the whole study as GSE322654_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1430815 and SRA study SRP680332. Searching any of these in the dataset finder brings you back here.

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