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Effects of nickel ion treatment on gene expression during osteogenic differentiation of mouse bone marrow mesenchymal stem cells

GSE322756 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/03 Platform GPL24247
Summary
Nickel (Ni) is a ubiquitous environmental trace metal, yet its physiological roles and dose-dependent effects in skeletal biology remain poorly understood. Through comprehensive in vivo and in vitro experiments, we demonstrated that low-dose Ni (0.1 μM) significantly enhances matrix mineralization during osteogenic differentiation, whereas this pro-mineralization effect is markedly attenuated at higher concentrations. Although the inhibitory mechanism of high-dose nickel on osteogenesis has been largely attributed to oxidative stress, the molecular basis underlying the promotional effect of low-dose nickel remains unclear. To address this, we performed RNA-seq analysis on primary mouse bone marrow mesenchymal stem cells (mBMSCs) treated with 0.1 μM nickel following 7 days of osteogenic induction, aiming to elucidate the downstream signaling pathways responsible for its beneficial effects on bone mineralization.
Published in
Dose-Dependent Effects of Nickel on Skeletal Development: Physiological Necessity and the Threshold of Toxicity
Ma X, Huang X, Li J et al. · International journal of molecular sciences 2026 · PMID 42196516 · doi:10.3390/ijms27104538
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Also filed as BioProject PRJNA1431414 and SRA study SRP680671. Searching any of these in the dataset finder brings you back here.

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