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Single-cell and Spatial Transcriptomic Profiling of the Bone Metastatic Microenvironment

GSE323357 Mus musculus Other; Expression profiling by high throughput sequencing 5 samples Submitted 2026/03/11 Platform GPL24247
Summary
The goal of this study is to comprehensively characterize the cellular composition, transcriptional programs, and spatial organization of the bone metastatic microenvironment and to assess how therapeutic perturbation influences tumor–microenvironment interactions. A mouse bone metastasis model was established via internal iliac artery injection of Lewis lung carcinoma cells, and metastatic lesions were monitored by in vivo imaging. Single-cell RNA sequencing and 10x Genomics Visium–based spatial transcriptomics were generated from metastatic bone tissues to provide complementary cellular and spatial resolution.
Published in
iS2C2: a cointelligent platform for mechanistic discovery of disease cellular crosstalk
Sheng J, Ahn JY, Yang L et al. · Signal transduction and targeted therapy 2026 · PMID 42108258 · doi:10.1038/s41392-026-02691-8
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Also filed as BioProject PRJNA1432110 and SRA study SRP681060. Searching any of these in the dataset finder brings you back here.

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