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Single-cell RNA-seq profiling of the DHT-induced androgenetic alopecia (AGA) mouse model treated with anti-DKK3 antibody

GSE325671 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/03/23 Platform GPL24247
Summary
Androgenetic alopecia (AGA) is characterized by progressive hair follicle miniaturization and remodeling of the skin microenvironment. To investigate cellular changes associated with AGA and the effects of DKK3 blockade, we performed single-cell RNA sequencing (scRNA-seq) of dorsal skin from a DHT-induced mouse model. Three experimental groups of male C3H/HeN mice were analyzed: vehicle control, DHT-treated AGA model, and DHT-treated mice receiving anti-DKK3 neutralizing antibody. Single-cell transcriptomic profiling identified cell population changes associated with DHT exposure and their modulation by anti-DKK3 treatment. This dataset provides a single-cell atlas of dorsal skin in the AGA model and following DKK3 inhibition.
Published in
DKK3-CKAP4 signaling drives fibroimmune remodeling and hair follicle miniaturization in androgenetic alopecia
An S, Kim H, Zheng M et al. · Theranostics 2026 · PMID 42370170 · doi:10.7150/thno.135412
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Direct links to NCBI, no account and no request form: the whole study as GSE325671_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 3 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1441450 and SRA study SRP685908. Searching any of these in the dataset finder brings you back here.

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