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Effects of LPS-induced neutrophil senescence on gene expression

GSE326268 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/04/02 Platform GPL24676
Summary
Neutrophils are the most abundant leukocytes in human peripheral blood but exhibit considerable heterogeneity, with distinct subsets characterized by diverse functional states. Lipopolysaccharide (LPS) has been shown to drive neutrophil senescence, a process distinct from apoptosis. However, the global transcriptional landscape of LPS-induced aged neutrophils remains largely unexplored. Here, we performed whole-transcriptome RNA sequencing (RNA-seq) on primary human neutrophils isolated from healthy donors. Neutrophils were treated with LPS to induce senescence, with untreated cells serving as controls. Our comparative transcriptomic analysis revealed that senescent neutrophils display significant upregulation of multiple genes associated with neutrophil extracellular trap (NET) formation compared to non-aged neutrophils. This dataset provides a comprehensive resource for investigating the molecular signatures underlying neutrophil senescence and its potential implications in inflammation and host defense.
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Direct links to NCBI, no account and no request form: the whole study as GSE326268_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1445035 and SRA study SRP687627. Searching any of these in the dataset finder brings you back here.

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