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Heat shock factor 5 is indispensable for pachynema progression and LINE1 silencing during spermatogenesis in mice [RNA-seq]

GSE327567 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/04/14 Platform GPL34290
Summary
Pachytene spermatocytes were isolated from adult wild-type and Hsf5 knockout mouse testes using STA-PUT velocity sedimentation. Single-cell suspensions were prepared by enzymatic digestion of seminiferous tubules with collagenase and trypsin. Cells were loaded onto a 4%–2%–0.5% BSA gradient and allowed to sediment for 1 hour and 45 minutes at 4°C. Fractions were collected, and pachytene spermatocytes were identified by SYCP3, γH2AX, and DAPI staining under microscopy. Enriched pachytene cells were pooled and processed for RNA-Seq.
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Also filed as BioProject PRJNA1451226 and SRA study SRP690644. Searching any of these in the dataset finder brings you back here.

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