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Transcriptional landscape of mouse adipose tissue transfected with LHX8 siRNA

GSE329222 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/21 Platform GPL23479
Summary
We have studied the role of LHX8 (Lim Homeobox 8) in adipocyte development. Mouse 3T3-L1 adipcoytes were used for our analyses. Cells were cultured in high glucose DMEM, supplemented with 10% FCS, 1% glutamine and 1% penicillin/streptomycin. Cells were grown to reach 70-80% confluence, and transfected with a siRNA silencer to decrease Lhx8 mRNA expression. Turbofectamine was used for cell transfection. Total RNA was isolated 48h post-transfection and used for subsequent NGS analysis.
Published in
Lim Homeobox 8 Is Essential for Beta Adrenergic Stimulation of Thermogenesis in Human Adipocytes
Gyurina K, Radványi Á, Sasi-Szabó L et al. · Cells 2026 · PMID 42274594 · doi:10.3390/cells15111000
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Also filed as BioProject PRJNA1458173 and SRA study SRP695277. Searching any of these in the dataset finder brings you back here.

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