GEO series

Long-term maintenance of H3K27me3 in postmitotic neurons is dispensable for gene expression regulation [ATAC-seq]

GSE330143 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 10 samples Submitted 2026/05/12 Platform GPL30172Platform GPL19057

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 10 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Mus musculus (mouse)
Samples: 10 samples: 3 × GC, 7m, WT, vs 3 × GC, 7m, Ezh1cKO, vs 2 × GCP, P5, vs 2 × GC, P21,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Neuronal maturation is associated with extensive changes in gene expression and 3D chromatin architecture. However, the molecular mechanisms that control the epigenetic landscape in terminally differentiated neurons are poorly understood. Here, we show that maturing neurons undergo a striking increase in the levels of the repressive histone modification H3K27me3 broadly across the genome.

Read the full summary ↓

Using mice with a conditional deletion in the catalytic domain of EZH1, the primary H3K27 methyltransferase in postmitotic neurons, we show that H3K27me3 maintenance depends on EZH1. Strikingly, an almost complete loss of H3K27me3 in postmitotic neurons induces minimal changes in gene expression and chromatin accessibility at 7 months of age. The amino acid composition of EZH1 suggests reduced sensitivity to H3K36 methylation, resulting in distinct activities of EZH1 and EZH2 in different chromatin contexts. Together, our results show that a postmitotic switch from EZH2 to EZH1 establishes novel chromatin domains in neurons with a minimal role in transcriptional maintenance.

This dataset

Open GSE330143 in finder → Find more mouse ChIP / ATAC / CUT&Tag datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE330143_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1462817 and SRA study SRP698098. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

3 × GC, 7m, WT, vs 3 × GC, 7m, Ezh1cKO, vs 2 × GCP, P5, vs 2 × GC, P21,

Supports a between-group comparison across 10 samples.

How we read this: 4 replicated groups read from 10 sample titles; they account for 10 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9719126 GCP, P5, rep1 GCP, P5,
GSM9719127 GCP, P5, rep2 GCP, P5,
GSM9719128 GC, P21, rep1 GC, P21,
GSM9719129 GC, P21, rep2 GC, P21,
GSM9719130 GC, 7m, WT, rep1 GC, 7m, WT,
GSM9719131 GC, 7m, WT, rep2 GC, 7m, WT,
GSM9719132 GC, 7m, WT, rep3 GC, 7m, WT,
GSM9719133 GC, 7m, Ezh1cKO, rep1 GC, 7m, Ezh1cKO,
GSM9719134 GC, 7m, Ezh1cKO, rep2 GC, 7m, Ezh1cKO,
GSM9719135 GC, 7m, Ezh1cKO, rep3 GC, 7m, Ezh1cKO,

Datasets on a similar topic

GSE330241 Long-term maintenance of H3K27me3 in postmitotic neurons is dispensable for gene expression regulation [ChIP-Seq] 16 samples shares h3k27me3, maintenance, postmitotic, dispensable, long-term, neurons
GSE329815 Long-term maintenance of H3K27me3 in postmitotic neurons is dispensable for gene expression regulation 44 samples shares h3k27me3, maintenance, postmitotic, dispensable, long-term, neurons
GSE190102 Ageing-Related Changes to H3K4me3, H3K27ac and H3K27me3 in Purified Mouse Neurons 43 samples shares h3k27me3, neurons
GSE225240 Reevaluation by CRISPR-Cas9 approach reveals multiple lincRNAs dispensable for pluripotency maintenance and SNORA73 essential for pluripotency exit 13 samples shares maintenance, dispensable
GSE225237 Reevaluation by CRISPR-Cas9 approach reveals multiple lincRNAs dispensable for pluripotency maintenance and SNORA73 essential for pluripotency exit (RNA-Seq) 12 samples shares maintenance, dispensable
GSE262390 Setd2 overexpression rescues bivalent gene expression during SCNT-mediated ZGA by enhancing H3K36me3 at gene bodies and excluding H3K27me3 from promoters [ChIP-seq] 79 samples shares h3k27me3
GSE255571 Chromatin remodeler CHD7 regulates neurogenic gene expression via controlling chromatin accessibility and modifications of histone marks H3K27me3 and H3K4me3 [Mouse CUT&Tag] 10 samples shares h3k27me3
GSE278811 EZHIP-mediated PRC2 inhibition is required to maintain H3K27me3-dependent imprinting post fertilization [ChIP-Seq] 77 samples shares h3k27me3

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