Comparison of FOLR2-negative and FOLR2-positive human decidual macrophages at term

Despite this, macrophage heterogeneity within the decidua remains poorly defined, with multiple classification systems proposed but no consensus regarding their biological relevance or application across gestation and labour. This thesis investigates decidual macrophage heterogeneity and function in decidua parietalis using flow cytometry, bulk RNA sequencing, RT-qPCR, ELISA, and functional tissue culture assays. Initial studies sought to validate a labour-associated macrophage population identified by single-cell RNA sequencing. Although a population with a similar phenotype was identified by flow cytometry, its extremely low abundance suggested it was unlikely to be a major driver of labour-associated immune adaptation. This prompted a broader investigation of decidual macrophage phenotyping strategies. Current and historical macrophage classification systems, including M1/M2 and several CD11c-based frameworks, failed to reliably distinguish macrophage subsets within this dataset. In contrast, a phenotyping strategy incorporating CD14, pan macrophage marker CD68, and tissue residency marker FOLR2 consistently identified monocytes, FOLR2- macrophages, and FOLR2+ macrophages across first trimester and term decidua, within both decidua parietalis and decidua basalis, and across term not in labour (TNL), term early labour (TeaL), and term in labour (TIL) samples. Bulk RNA sequencing validated FOLR2- and FOLR2+ macrophages as transcriptionally distinct populations. FOLR2- macrophages were enriched for pathways associated with neutrophil chemotaxis, antimicrobial responses, and inflammation, whereas FOLR2+ macrophages were enriched for antigen presentation, MHC class II assembly, and tissue-resident functions. Labour was associated with selective remodelling of the decidual myeloid compartment rather than global leukocyte infiltration. Monocyte abundance increased significantly during early labour, while the proportion of FOLR2- macrophages decreased before increasing again in established labour. Functional analyses demonstrated distinct cytokine profiles between macrophage subsets. FOLR2- macrophages preferentially expressed IL-8 and increased CCL5 expression during early labour, consistent with a chemotactic and inflammatory phenotype. In contrast, FOLR2+ macrophages exhibited high expression of IL-6, CCL2, MMP-9, and MMP-19, supporting roles in tissue homeostasis, leukocyte recruitment, and extracellular matrix remodelling. Furthermore, co-culture experiments demonstrated that FOLR2+ macrophages promoted degradation of chorionic collagen in vitro. Together, these findings support a model in which labour-associated immune adaptation is mediated by functionally specialised decidual macrophage populations rather than a single labour-specific macrophage subset. Functional analysis of FOLR2- macrophages suggests they contribute predominantly to inflammatory signalling and leukocyte recruitment, whereas FOLR2+ macrophages contribute to antigen presentation and tissue remodelling. This work establishes a simple and robust framework for decidual macrophage phenotyping that is applicable across gestation, decidual compartment, and labour status, providing a foundation for future studies investigating macrophage function at the maternal-fetal interface.