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CRISPR activation of DLX5 drives neural progenitors to the GnRH cell fate

GSE337839 Homo sapiens (human) RNA-seq (gene expression) 29 samples Submitted 2026/07/29 Platform GPL34284
At a glance

RNA-seq (gene expression) · human · 29 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
29 samples: 7 conditions, mostly with about 5 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
CRISPR activation of DLX5 drives neural progenitors to the GnRH cell fateJournal of molecular endocrinology · 2026
Summary (written by the submitters)
Gonadotropin-releasing hormone (GnRH) neurons regulate the hypothalamic pituitary-gonadal axis and are required for puberty onset and reproductive competence. However, the transcriptional regulators governing GnRH neuron specification and migration remain poorly defined. The homeodomain transcription factor DLX5 is expressed in fetal human GnRH neurons, its expression precedes that of GNRH1 in human pluripotent stem cell (hPSC)-derived GnRH neurons, and in mice it serves as a guidance cue for GnRH neuron migration.
Read the full summary ↓
We hypothesized that DLX5 may act as an upstream regulator of human GnRH neuron fate specification and migratory capacity. Using CRISPR activation, we upregulated DLX5 during FGF8b-directed differentiation of hPSCs to GnRH neurons via dual SMAD inhibition and Notch inhibition, as previously described. DLX5 activation increased neural progenitor motility (p<0.001), upregulated FGF8 (p<0.05), and induced GABAergic markers including GAD1 and GAD2. Notably, DLX5 activation induced GNRH1 in the absence of exogenous FGF8b (p<0.05), suggesting that, in GnRH neurons, DLX5 regulates FGF8. When combined with exogenous FGF8b, DLX5 activation produced distinct neuronal patterning accompanied by upregulation of extracellular matrix genes such as SPARC, which has been implicated in neurite outgrowth. Collectively, these data indicate that activation of DLX5 promotes GnRH neurogenesis from hPSCs by driving GABAergic fate, inducing FGF8, and remodelling the extracellular matrix.
Published in
CRISPR activation of DLX5 drives neural progenitors to the GnRH cell fate
Madhusudan S, Eskici N, Gomez-Sanchez C et al. · Journal of molecular endocrinology 2026 · PMID 42454502 · doi:10.1530/JME-26-0040
This dataset
Open GSE337839 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE337839_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1491323 and SRA study SRP715955. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42454502) ↗
Study design
7 conditions, mostly with about 5 replicates each
WA09 cells, Day20, 100 ng/ml FGF8b, not DLX5 … ×5 WA09 cells, Day25, 100 ng/ml FGF8b, not DLX5 … ×5 WA09 cells, Day25, 100 ng/ml FGF8b, DLX5 acti… ×5 WA09 cells, Day20, 100 ng/ml FGF8b, DLX5 acti… ×4 WA09 cells, Day25, FACS-sorted TdT positive n… ×3 WA09 cells, Day25, FACS-sorted TdT positive n… ×3 WA09 cells, Day25, FACS-sorted TdT positive n… ×3 WA09 cells, Day20, 100 ng/ml FGF8b, DLX5 acti… ×1

Supports a between-group comparison across 28 samples.

How we read this: 7 replicated groups read from 29 sample titles; they account for 28 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.