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Single-cell RNA-Seq reveals dynamic, random monoallelic gene expression in mammalian cells

GSE45719 Mus musculus Expression profiling by high throughput sequencing 317 samples Submitted 2014/01/10 Platform GPL13112
Summary
In the diploid genome, genes come in two copies, which can have different DNA sequence and where one is maternal and one is paternal. In a particular cell, a gene could potentially be expressed from both copies (biallelic expression) or only one (monoallelic). We performed RNA-Sequencing on individual cells, from zygote to the cells of the late blastocyst, and also individual cells from the adult liver. Using first generation crosses between two distantly related mouse strains, CAST/Ei and C57BL/6, we determined the expression separately from the maternal and paternal alleles. We found that half of the genes were expressed by only one allele, randomly so that some cells would express the paternal allele, some the maternal and a few cell both alleles. We also observed the spread of the progressive inactivation of the paternal X chromosome.
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Direct links to NCBI, no account and no request form: the whole study as GSE45719_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 317 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA195938 and SRA study SRP020490. Searching any of these in the dataset finder brings you back here.

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