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ALKBH1-Mediated Transfer RNA Demethylation

GSE65299 Homo sapiens; Mus musculus Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing 9 samples Submitted 2016/10/13 Platform GPL16791Platform GPL17021
Summary
Transfer RNA (tRNA) is a central component of protein synthesis and cell signaling network. One salient feature of tRNA is its heavily modified status, which can critically impact its function. Here we show that mammalian ALKBH1 is a tRNA demethylase. It mediates the demethylation of N1-methyladenosine (m1A) in tRNAs. The ALKBH1-catalyzed demethylation of the target tRNAs results in their reduced partition in polysomes and their decreased usage in protein synthesis. This process is dynamic and responds to glucose availability to affect translation. Our results uncover reversible methylation of tRNA as a new regulatory mechanism of post-transcriptional gene expression.
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Also filed as BioProject PRJNA273642 and SRA study SRP052831. Searching any of these in the dataset finder brings you back here.

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