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Transcriptomics analysis of gene expression in 10 embryo development stages of Danio rerio

GSE65556 Danio rerio Expression profiling by high throughput sequencing 10 samples Submitted 2018/02/01 Platform GPL14875
Summary
RNA was isolated from Danio embryo cells using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) of higher than 9. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq2000 (Illumina) in single-read mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane.
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Direct links to NCBI, no account and no request form: the whole study as GSE65556_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 10 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA274362 and SRA study SRP053099. Searching any of these in the dataset finder brings you back here.

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