GEO series

Brown and white adipocyte differentiation

GSE7032 Mus musculus (mouse) Expression microarray 24 samples Submitted 2007/05/31 Platform GPL81

At a glance

Expression microarray · mouse · 24 samples

What it is: Expression microarrayGene activity read on a chip (the older technology that RNA-seq replaced).
Organism: Mus musculus (mouse)
Samples: 24 samples
Data you can get: A processed expression table (series matrix) and the array files, from NCBI.

Summary (written by the submitters)

Attainment of a brown adipocyte cell phenotype in white adipocytes, with their abundant mitochondria and increased energy expenditure potential, is a legitimate strategy for combating obesity. The unique transcriptional regulators of the primary brown adipocyte phenotype are unknown, limiting our ability to promote brown adipogenesis over white.

Read the full summary ↓

In the present work, we used microarray analysis strategies to study primary preadipocytes, and we made the striking discovery that brown preadipocytes demonstrate a myogenic transcriptional signature, whereas both brown and white primary preadipocytes demonstrate signatures distinct from those found in immortalized adipogenic models. We found a plausible SIRT1-related transcriptional signature during brown adipocyte differentiation that may contribute to silencing the myogenic signature. In contrast to brown preadipocytes or skeletal muscle cells, white preadipocytes express Tcf21, a transcription factor that has been shown to suppress myogenesis and nuclear receptor activity. In addition, we identified a number of developmental genes that are differentially expressed between brown and white preadipocytes and that have recently been implicated in human obesity. The interlinkage between the myocyte and the brown preadipocyte confirms the distinct origin for brown versus white adipose tissue and also represents a plausible explanation as to why brown adipocytes ultimately specialize in lipid catabolism rather than storage, much like oxidative skeletal muscle tissue. Keywords: In vitro differentiation

This dataset

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Download · which file do I need?

Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗

3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE7032_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

Whole study · RAW.tar ↓

What the files usually look like: For arrays the series matrix is normally the normalised expression table you want; the raw array files (CEL and similar) are in the RAW.tar.

Also filed as BioProject PRJNA98391. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 17360536) ↗

Samples in this study

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+ 24 more — browse all 24 samples with per-sample file links →

Datasets on a similar topic

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GSE328946 Sphingosine kinase 2 regulates adipocyte browning and whole-body metabolism 36 samples shares adipocyte, brown
GSE218943 Transcriptional profiling of mature murine adipocytes of the inguinal white adipose tissue depot following LPCAT3 deletion and dietary PUFA manipulation 30 samples shares adipocyte, white
GSE232928 Transcriptional profiling of mature murine adipocytes of the inguinal white adipose tissue depot following LPCAT3 deletion and HFD-induced adipose hypertrophy. 24 samples shares adipocyte, white
GSE242882 CPEB2-activated Prdm16 translation promotes brown adipocyte function and prevents obesity 22 samples shares adipocyte, brown
GSE293584 RNA-sequencing of SVF-derived preadipocytes and adipocytes from inguinal white adipose tissue of male mice treated with vehicle, CGRPa, NPY, NE, or SP 21 samples shares adipocyte, white
GSE291567 Multi-omics analysis of thermogenic lipolysis in brown adipocytes 18 samples shares adipocyte, brown

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