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Patient-derived organoid cultures model human primary liver cancer in vitro

GSE84073 Homo sapiens Expression profiling by high throughput sequencing; Other 59 samples Submitted 2017/11/13 Platform GPL18460Platform GPL20301
Summary
The study of human liver cancer has been hampered by the lack of reliable models that faithfully recapitulate the physiopathology of the patient’s original tumour ex vivo. Here, we describe the first culture system to allow the establishment and long-term expansion of human primary liver cancer (PLC) organoids (called tumoroids) from the three most common PLC subtypes: Hepatocellular carcinoma (HCC), Cholangiocarcinoma (CC) and mixed HCC/CC tumours (CHC). We evaluated whether tumoroid lines maintain the expression profile and the genomic landscape of the original tumor they derived from by performing genome-wide (RNAseq/WES) analyses before and after culture. These analyses reveal that the cultures closely recapitulate the gene expression and the mutational landscape of the original tumor, allowing discrimination between different tumor subtypes (HCC, CHC, CC).
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Direct links to NCBI, no account and no request form: the whole study as GSE84073_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 59 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA327939 and SRA study SRP077971. Searching any of these in the dataset finder brings you back here.

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