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The dynamics of cellular response to therapeutic perturbation using multiplexed quantification of the proteome and transcriptome at single-cell resolution

GSE100501 Homo sapiens Expression profiling by high throughput sequencing; Other 31 samples Submitted 2017/08/28 Platform GPL16791
Summary
We developed a scalable assay permitting the simultaneous quantification of hundreds of proteins and the full transcriptome in thousands of individual cells from samples where cell number is limiting. The RNA Expression and Protein Sequencing assay (REAP-seq) uses DNA-labeled antibodies to permit DNA sequencing of both mRNA and antibodies in a single workflow using droplet microfluidics. We describe the development and validation of REAP-seq in human PBMCs, and use the assay to assess the costimulatory effects of an anti-CD27 agonist antibody in naïve CD8+ lymphocytes. Protein quantification using REAP-seq was more sensitive than parallel mRNA measurements and differentiated cell states with fewer analytes. Unbiased profiling of single cells without prior selection enabled identification and characterization of an unexpected cell type that would have been missed with population-averaged profiling methodologies.
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Direct links to NCBI, no account and no request form: the whole study as GSE100501_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 31 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA391958 and SRA study SRP110537. Searching any of these in the dataset finder brings you back here.

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