Bulk RNA Sequencing of Wild Type and Cisplatin Induced AKI (3d and 7d groups) Mouse Kidney

The study of transcriptome analyses using the Illumina HiSeq 4000 platform for renal tissue from three groups (Sham, 3 and 7 days after cisplatin induced AKI, n=3). In total, over 66.2 Gb clean reads were produced; Differentially expressed gene analysis identified 3984 and 2139 genes which were expressed higher or lower in cis3d group when compared to Sham group. Among which, injury related genes, such as Havcr1, Lcn2, krt20 were significantly increased in cis3d group. On the other hand, the scale of different genes between cis7d and sham groups were greatly shrunk to 2144 (1564 up regulated and 580 down regulated). Accordingly, both the cell injury markers (krt20, lcn2 and havcr1) were markedly restored in cis7d group. In brief, the result of sham, cis3d and cis7d group shown the gene expression transition of injury pattern (progression stage) to repair pattern (regression stage) from 3 days to 7 days after acute injury of kidney tissue.