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Single nuclei RNA sequencing of skin draining lymph nodes in the setting of skin inflammation and RAG1 fate mapping

GSE192597 Mus musculus Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2024/04/28 Platform GPL24247
Summary
Type 2 inflammation contributes to the pathology of skin diseases such as atopic dermatitis (AD) and urticaria. We previously found that AD-like inflammation induced by calcipotriol in mice is critically mediated by group 2 innate lymphoid cells (ILC2s), while T cells and B cells are dispensable given development of robust AD-like disease in RAG knockout mice. Indeed, we found that in RAG knockout mice AD-like inflammation was worse, with a proportion of ILC2s displaying increased markers of activation. The goal of this study was to characterize transcriptional and epigenomic changes in ILC populations with a history, or not, of expressing RAG1 in the setting of AD-like skin inflammation.
Published in
RAG suppresses group 2 innate lymphoid cells
Ver Heul AM, Mack M, Zamidar L et al. · bioRxiv : the preprint server for biology 2025 · PMID 38712036 · doi:10.1101/2024.04.23.590767
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Also filed as BioProject PRJNA792191 and SRA study SRP352416. Searching any of these in the dataset finder brings you back here.

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