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High-throughput sequencing for analysis of macropahges from old and young mice.

GSE198666 Mus musculus Expression profiling by high throughput sequencing 25 samples 2024/05/31 GPL17021GPL21103
Summary
Immune cells are essential for proper fracture healing. The goal of this study was to compare the transcriptional profiles of the CD45+ immune cells isolated from the healing fracture callus of old (24 month) and young (3 month) mice (C57BL/6). Analysis of all CD45+ cells was completed via single cell RNAseq. Additional analysis of the macrophages involved in fracture healing in old and young mice was performed via bulk RNAseq. We show that there were multiple subpopulations of macrophages that were transcriptionally heterogenous and present simultaneously within the fracture callus tissue. Attenuated healing in the old mice was associated with a transcriptional profile that shifted towards more pro-inflammatory phenotypes.
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NCBI GEO page ↗ Paper (PMID 38825965) ↗ {# Names what the click gives you. "Open in finder" meant nothing to a visitor who arrived from a search engine and has never seen the tool. #} Find more mouse RNA-seq datasets →
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