GEO series

Mechanical force coordinates with estrogen to orchestrate alveolar bone homeostasis via PIEZO1

GSE205377 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 10 samples Submitted 2025/12/31 Platform GPL24247

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 10 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Mus musculus (mouse)
Samples: 10 samples: 3 × C3H10_Ctrl, vs 3 × C3H10_CMS, vs 2 × C3H10_GFP, vs 2 × C3H10_Piezo1 knock out,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Healthy alveolar bone is the cornerstone for oral function and oral treatment. In the aging population, increasingly postmenopausal women requiring dental services experiences alveolar bone loss due to estrogen deficiency. Both local factors (occlusal force lose) and systemic factors (estrogen deficiency) affects their alveolar bone health, but the association remains to be clarified.

Read the full summary ↓

Here, we identified that occlusal force is critical in maintaining alveolar bone mass and PIEZO1 coordinates mechanical force and estrogen, thus orchestrating alveolar bone homeostasis. Mechanistically, both occlusal force loss and PIEZO1 deletion may impair the osteogenesis of alveolar bone and sequentially regulate catabolic metabolism through Fas ligand (FasL)-induced osteoclastic apoptosis. PIEZO1 may promote the activity of signal transducer and activator of transcription 3 (STAT3) and estrogen receptor 1 (ESR1), which cooperatively increase Fasl transcription. This study thus revealed a cooperation between occlusal force and estrogen in maintaining alveolar bone homeostasis through a PIEZO1-STAT3/ESR1-FasL pathway. Our work supports restoration of occlusal force with dental therapies early and suggests that preventing alveolar bone loss is the major priority. PIEZO1 may serve not only as a treatment target for occlusal force loss-induced alveolar bone loss but also as a potential target for metabolic bone loss, especially in older patients.

This dataset

Open GSE205377 in finder → Find more mouse ChIP / ATAC / CUT&Tag datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE205377_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA844902 and SRA study SRP378197. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

3 × C3H10_Ctrl, vs 3 × C3H10_CMS, vs 2 × C3H10_GFP, vs 2 × C3H10_Piezo1 knock out,

Supports a between-group comparison across 10 samples.

How we read this: 4 replicated groups read from 10 sample titles; they account for 10 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM6211148 C3H10_GFP,replicate 1 C3H10_GFP,
GSM6211149 C3H10_GFP,replicate 2 C3H10_GFP,
GSM6211150 C3H10_Piezo1 knock out,replicate 1 C3H10_Piezo1 knock out,
GSM6211151 C3H10_Piezo1 knock out,replicate 2 C3H10_Piezo1 knock out,
GSM6556244 C3H10_Ctrl,replicate 1 C3H10_Ctrl,
GSM6556245 C3H10_Ctrl,replicate 2 C3H10_Ctrl,
GSM6556246 C3H10_Ctrl,replicate 3 C3H10_Ctrl,
GSM6556247 C3H10_CMS,replicate 1 C3H10_CMS,
GSM6556248 C3H10_CMS,replicate 2 C3H10_CMS,
GSM6556249 C3H10_CMS,replicate 3 C3H10_CMS,

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