Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Fndc1
RNA-seq (gene expression) · mouse · 10 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 10 samples: 5 × nc vs 5 × fndc1Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- FNDC1 is a myokine that promotes myogenesis and muscle regenerationThe EMBO journal · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE217125_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA896977 and SRA study SRP405943. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 10 samples.
How we read this: 2 replicated groups read from 10 sample titles; they account for 10 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM6705454 nc1 nc
- GSM6705455 nc2 nc
- GSM6705456 nc3 nc
- GSM6705457 nc4 nc
- GSM6705458 nc5 nc
- GSM6705459 fndc1_1 fndc1
- GSM6705460 fndc1_2 fndc1
- GSM6705461 fndc1_3 fndc1
- GSM6705462 fndc1_4 fndc1
- GSM6705463 fndc1_5 fndc1
- GSE194370 RNAseq of 3 and 5 days differentiated C2C12 cells treated with conditioned medium of KPC, 4662, LLC or C26 cells 42 samples shares c2c12, treated
- GSE333910 Transcriptomic profiling of C2C12 myotubes treated with D-galactose and red ginseng-derived interventions 30 samples shares c2c12, treated
- GSE316686 Total RNA-seq of C2C12 myotubes treated with agrin or MuSK antibodies 20 samples shares c2c12, treated
- GSE294869 The effect of carnitine treatment on palmitic acid-treated C2C12 myotubes 9 samples shares c2c12, treated
- GSE249627 RNA sequencing of mouse myoblast cells C2C12 with and without lenvatinib treated after 4 days differentiated. 6 samples shares c2c12, treated
- GSE49535 Effect of H19 Knockdown by siRNA on gene expression in C2C12 cells 6 samples shares c2c12, sirna
- GSE284614 Tumor-site directed A1R expression enhances CAR T cell function and improves efficacy against solid tumors. [Mouse RNAseq constitutive] 9 samples shares directed, against
- GSE286003 RNA-seq of sipinal cord of WT, AR-24Q, AR-97Q, testosterone-treated AR-97Q mice, Scramble-ASO-treated AR-97Q mice, and Rest4-ASO #3-treated AR-97Q mice at P7 18 samples shares scramble, treated
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.