Gene expression data at the single cell level of human hamstring, deltoid, supraspinatus muscle; as well as, cultured human muscle fibroadipogenic cells (FAPs)
Single-cell RNA-seq · human · 17 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
- Organism
- Homo sapiens (human)
- Samples
- 17 samples: 17 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE227548_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA945484 and SRA study SRP427768. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 17 sample titles: 17 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7102545 muscle, cuff, 42_cuff
- GSM7102546 muscle, delt, 42_delt
- GSM7102547 muscle, cuff, 43m_cuff
- GSM7102548 muscle, delt, 43m_delt
- GSM7102549 muscle, cuff, 48f_cuff
- GSM7102550 muscle, delt, 48f_delt
- GSM7102551 muscle, cuff, 56m_cuff
- GSM7102552 muscle, delt, 56m_delt
- GSM7102553 muscle, cuff, 72_cuff
- GSM7102554 muscle, delt, 72_delt
- GSM7102555 muscle, cuff, 79f_cuff
- GSM7102556 muscle, delt, 79f_delt
- GSM7102557 muscle, ham, 24m_ham
- GSM7102558 muscle, ham, 32m
- GSM7102559 FAPs, adipogenic culture, adipo
- GSM7102560 FAPs, standard culture, std
- GSM7102561 FAPs, fibrogenic culture, fibro
- GSE278505 Krill oil supplementation in vivo promotes increased fuel metabolism and protein synthesis in cultured human skeletal muscle cells 16 samples shares cultured, muscle
- GSE270681 Effect of elastin insufficiency on gene expression in cultured human aortic smooth muscle cells 6 samples shares cultured, muscle
- GSE225177 Gene expression profile at single cell level of human peripheral blood mononuclear cells cocultured with rotavirus treated or PBS treated human cholangiocyte cell line H69. 2 samples shares cultured, level
- GSE233137 Gene expression profile at single cell level of cultured normal human epidermal melanocytes (NHEMs) [NHEM_FSK_scRNA] 2 samples shares cultured, level
- GSE316799 Gene expression profile at single cell level of human bronchial epithelium cells cultured at the air-liquid interface (ALI) treated with CdCl2 (10µM) or DMSO (0,1%) 1 samples shares cultured, level
- GSE184487 RNA-sequencing of PBMC cultured with PolyI:C or LPS, from a pediatric cohort who received OM85 or placebo. 193 samples shares cultured
- GSE175354 Gene expression analysis of myeloid cells cultured in the presence of fibronectin and/or anti-ILT3 16C5 72 samples shares cultured
- GSE269688 Molecular effects of arsenic and smokeless tobacco aqueous extract in cultured cells [RNA-seq] 44 samples shares cultured
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.