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NKAPL binding sites in mouse testis and the genome-wide occupancy of the SOX30/HDAC3 protein complex and RNA Pol II in the absence of NKAPL

GSE232398 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 20 samples Submitted 2024/12/12 Platform GPL24247
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 20 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Mus musculus (mouse)
Samples
20 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
NKAPL facilitates transcription pause-release and bridges elongation to initiation during meiosis exitNature communications · 2025
Summary (written by the submitters)
To profile genomic binding sites of NKAPL and examine whether its knockout alters the genome-wide occupancy of the SOX30/HDAC3 protein complex and RNA Pol II in mouse testes, wild-type and Nkapl KO testes at postnatal day 21 were harvested for ChIP-seq.
Published in
NKAPL facilitates transcription pause-release and bridges elongation to initiation during meiosis exit
Kang Z, Xu C, Lu S et al. · Nature communications 2025 · PMID 39824811 · doi:10.1038/s41467-024-55579-y
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE232398_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA971928 and SRA study SRP437436. Searching any of these in the dataset finder brings you back here.

Study design
6 conditions, mostly in duplicate
HDAC3_WT ×2 SOX30_WT ×2 WT_PS_Pol ll ×2 WT_RS_Pol ll ×2 KO_PS_Pol ll ×2 KO_RS_Pol ll ×2 NKAPL_WT ×1 Pol ll_WT ×1 +6 more

Supports a between-group comparison across 12 samples.

How we read this: 6 replicated groups read from 20 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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